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Journal / Biology / Isolation of Retinal Stem Cells from the Mouse Eye
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Biology

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Isolation of Retinal Stem Cells from the Mouse Eye
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Isolation of Retinal Stem Cells from the Mouse Eye

Article DOI: 10.3791/2209-v • 07:23 min • September 11th, 2010
September 11th, 2010 •
1, 1
1Molecular Genetics, University of Toronto

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Summary
Automatic Translation

In this video, we will demonstrate how to isolate retinal stem cells from the ciliary epithelium of the mouse eye and grow them in culture to form clonal retinal spheres. The spheres that are isolated possess the cardinal properties of stem cells: self-renewal and multipotentiality.

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Isolation Retinal Stem Cells Mouse Eye Ciliary Epithelium Adult Mouse Quiescent Cell CE Pigmented Cell Non-pigmented Cell Neural Retina RPE Controversy Spheres Differentiation Regeneration Injury Mammalian Eye Stem Cell Identification Mechanisms Quiescence Transplantation Ciliary Epithelial Cells
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