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Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
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Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

Article DOI: 10.3791/2237 • 06:48 min • July 15th, 2010
July 15th, 2010 •
1, 1
1GIBCO, Life Technologies

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Summary
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This protocol describes the detailed procedure for cryopreserving human iPS cells in KnockOut SR cryopreservation medium and recovering these cells in complete KnockOut SR Feeder Free (KSR-FF) medium or feeder-based KnockOut SR medium.

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Cryopreserving Recovering Human IPS Cells Complete KnockOut Serum Replacement Feeder-free Medium Reprogramming Fibroblasts Pluripotent Cells Drug Discovery Cell Therapy Basic Research GIBCO Media And Reagents Knockout DMEM Knockout Serum Replacement Embryonic Stem Cell Growth IPS Cell Culture Feeder-free Culture Knockout SR Growth Factor Cocktail Traditional Culture Methods Mouse Or Human Fibroblast Feeder Layers Feeder-conditioned Medium Labor-intensive Hard To Scale Undifferentiated Cells
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