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Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

DOI:
10.3791/2315-v

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13:45 min

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December 23, 2010

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,
1Microbiology and Immunology, James Graham Brown Cancer Center,University of Louisville

Chapters

  • 00:05Title
  • 01:13Introduction
  • 01:48Preparation of Bone Marrow Derived Dendritic Cells (BMDC)
  • 02:42Preparation of Lipid Loaded Micropipette
  • 04:20Live-cell Imaging of Cell Migration in Response to Ligand Application
  • 05:25Representative Results for Migration of BMDCs
  • 06:03Live Cell Imaging of Transfected RBL-2H3 Cells
  • 09:49Image Analysis
  • 10:51Representative Results
  • 13:03Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describes the methodology to determine the chemotactic response of leukocytes to specific ligands and identify interactions between the cell surface receptors and cytosolic proteins using live cell imaging techniques.

Tags

Leukotriene B4Cell MigrationBLT1β-arrestinG-protein Coupled ReceptorsTransmembrane Protein FamilyExtracellular SignalsIntracellular ResponsesChemotaxisCalcium ReleaseGene RegulationLigand BindingConformational ChangesHeterotrimeric G-proteinsSecond MessengersCyclic Adenosine Monophosphate (cAMP)Inositol Triphosphate (IP3)Diacyl Glycerol (DG)DesensitizationSequestrationInternalizationG-protein Receptor Kinases (GRKs)Cytosolic ProteinsMembrane TranslocationPro-inflammatory Lipid MoleculeArachidonic Acid Pathway

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