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Recapitulation of an Ion Channel IV Curve Using Frequency Components
JoVE Journal
Biology
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JoVE Journal Biology
Recapitulation of an Ion Channel IV Curve Using Frequency Components
DOI:

10:14 min

February 08, 2011

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Chapters

  • 00:05Title
  • 01:29Prepare Noise Function and Input Signal
  • 03:10Perform Voltage Clamp Measurements
  • 03:55Data Analysis – IV Curves, FFTs, and Correlation Coefficients
  • 05:16Results: Model Cell Recordings and IV-Frequency Curve Correlations
  • 08:51Future Application to Multiple Channels in Biological Cells
  • 09:33Conclusion

Summary

Automatic Translation

There are technical obstacles to measuring current flux through multiple ion channels simultaneously, and later discerning what portion of the transmembrane current is due to each channel type. To address this need, this method presents a way to generate the IV curve of individual channel types using specific frequency components.

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