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January 11, 2011
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Here we describe an efficient strategy to remove the silk-producing glands from the abdomen of female black widow spiders. This procedure allows the rapid isolation of the seven distinct silk-producing glands in a highly purified fashion, an important process for investigators studying spider silk production and fiber assembly.
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Jeffery, F., La Mattina, C., Tuton-Blasingame, T., Hsia, Y., Gnesa, E., Zhao, L., Franz, A., Vierra, C. Microdissection of Black Widow Spider Silk-producing Glands. J. Vis. Exp. (47), e2382, doi:10.3791/2382 (2011).
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