Synergistic Photobiomodulation with a Dual-Wavelength Laser in an In Vitro Disease Model

Divide the transwell system into five groups that undergo various treatments.

The first group serves as the control and consists of a co-culture of neuroblastoma and microglia cells.

In the second group, treat the co-culturer with fibrillar β-amyloid (fAβ) alone. fAβ induces damage in neuroblastoma cells.

In the third group, treat neuroblastoma cells with an 808-nanometer laser, then co-culture them with untreated microglia.

The laser reduces oxidative stress, improving neuronal survival.

In the fourth group, treat microglia with a 1,064-nanometer laser, then co-culture them with untreated neuroblastoma cells.

Activated microglia enhance fAβ clearance and release cytokines, protecting neuroblastoma cells.

In the fifth group, expose microglia to a 1,064 nanometer laser and neuroblastoma cells to an 808 nanometer laser, then co-culture them.

The combined effects of dual-wavelength exposure lead to greater neuronal survival compared to single-wavelength treatments, demonstrating the neuroprotective potential of synergistic photobiomodulation therapy.