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Methods for Patch Clamp Capacitance Recordings from the Calyx
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Methods for Patch Clamp Capacitance Recordings from the Calyx

Methods for Patch Clamp Capacitance Recordings from the Calyx

DOI:
10.3791/244-v

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14:58 min

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July 29, 2007

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, ,
1National Institute of Neurological Disorders and Stroke,National Institute of Health

Chapters

  • 00:00Introduction
  • 00:25Dissection Overview
  • 02:00Dissection of Brain
  • 05:52Physiology Experiments
  • 11:34Locating an Accessible Calyx
  • 12:16After Some Searching
  • 14:04Conclusion
  • 14:48Credits

Summary

Automatic Translation

Automatic Translation

We demonstrate the basic techniques for presynaptic patch clamp recording at the calyx of Held, a mammalian central nervous system nerve terminal.

Tags

Patch ClampCapacitance RecordingsCalyx Of HeldPresynaptic TerminalAction PotentialsCalcium Channel CurrentsVesicle FusionExocytosisMembrane UptakeEndocytosisCell MembraneNeurotransmitterCapacitance IncreaseCapacitance ReductionVesicular ReleaseMammalian CNS Nerve TerminalPostsynaptic ActivitySlice PreparationMorphological FeaturesPatch Clamping Techniques

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