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Detection of Functional Matrix Metalloproteinases by Zymography

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Detection of Functional Matrix Metalloproteinases by Zymography

Detection of Functional Matrix Metalloproteinases by Zymography

DOI:

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09:30 min

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November 08, 2010

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Xueyou Hu, Christine Beeton

¹Department of Molecular Physiology and Biophysics,Baylor College of Medicine

Chapters

00:00Title
00:54Introduction
01:23Zymography Gel Electrophoresis
03:43Renaturing and Developing the Gel
06:07Data Analysis
08:03Representative Results for the Detection of MMP-2 using Zymography
09:00Conclusion

Summary

Automatic Translation

This protocol describes an activity-based assay for detecting matrix metalloproteinases in culture supernatants or body fluids.

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