Journal
/
Immunology and Infection
/
RNA Interference in Ticks
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
RNA Interference in Ticks

RNA Interference in Ticks

DOI:
10.3791/2474-v

•

09:06 min

•

January 20, 2011

•

, ,
1Department of Veterinary Pathobiology, Center for Veterinary Health Sciences,Oklahoma State University, 2(CSIC-UCLM-JCCM),Instituto de Investigación en Recursos Cinegéticos IREC

Chapters

  • 00:00Title
  • 01:45Generation of dsRNA
  • 02:28Preparation and Injection of Ticks
  • 04:50Treatment of Ticks after Injection
  • 06:31Tick Analysis after RNAi
  • 07:34Representative Tick RNAi Results
  • 08:30Conclusion

Summary

Automatic Translation

Automatic Translation

A method for RNA interference (RNAi) by injection of dsRNA into unfed ticks is described. RNAi is the most widely used gene-silencing technique in ticks where the use of other methods of genetic manipulation has been limited.

Tags

RNA InterferenceTicksHematophagous EctoparasitesVectors Of Human DiseasesCattle IndustryControl Tick InfestationsRNAiGene Expression DisruptionSmall Interfering RNAs (siRNAs)Double-stranded RNA (dsRNA)Post-transcriptional Gene SilencingFunctional Genomics StudiesGenetic Manipulation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2474?article_type=v

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
check_url/2474?article_type=v

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks
check_url/2474?article_type=v

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research
check_url/2474?article_type=v

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
check_url/2474?article_type=v

Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
check_url/2474?article_type=v

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
check_url/2474?article_type=v

RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
check_url/2474?article_type=v

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
check_url/2474?article_type=v

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
check_url/2474?article_type=v

Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection

Read Article