Purification of Progenitors from Skeletal Muscle

Lin Yi; Fabio Rossi

doi:10.3791/2476

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Biology

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Purification of Progenitors from Skeletal Muscle

JoVE Journal
Biology

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JoVE Journal Biology

Purification of Progenitors from Skeletal Muscle

18,052 Views

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12:55 min

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March 16, 2011

DOI:

10.3791/2476-v

DOI:

10.3791/2476-v

•

12:55 min

March 16, 2011

•

7 Views

Lin Yi¹, Fabio Rossi¹

¹The Biomedical Research Centre,University of British Columbia

Summary

Method for the enzymatic dissociation, surface labeling and purification by flow cytometry of fibro/adipogenic and myogenic progenitors from murine skeletal muscle.

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Purification Progenitors Skeletal Muscle Myogenic Progenitors Satellite Cells Basement Membrane Vessel-associated Progenitors Myofibroblasts White Adipocytes Proliferation Trophic Support Assays Isolation Transplantation Flow Cytometry Enzymatic Dissociation Colony-forming Cells Cell Types Molecular Analysis

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Purification of Progenitors from Skeletal Muscle

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