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JoVE Journal
Biology

This content is Open Access.

Heterokaryon Technique for Analysis of Cell Type-specific Localization
 

Heterokaryon Technique for Analysis of Cell Type-specific Localization

Article
March 11th, 2011

Summary March 11th, 2011

A flexible and efficient method for the characterization of cell type-specific protein localization and nucleocytoplasmic shuttling is described. This heterokaryon approach uses fluorescently-labeled fusion proteins to image protein localizations after cell fusion. The protocol is amenable to steady-state localizations or more dynamic determinations based on live cell imaging.

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