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Imaging Cell Shape Change in Living Drosophila Embryos
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Imaging Cell Shape Change in Living Drosophila Embryos

Imaging Cell Shape Change in Living Drosophila Embryos

DOI:
10.3791/2503-v

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11:20 min

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March 30, 2011

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,
1Program in Cell & Molecular Biology,Baylor College of Medicine (BCM), 2Verna & Marrs McLean Department of Biochemistry & Molecular Biology,Baylor College of Medicine (BCM)

Chapters

  • 00:05Title
  • 00:45Introduction
  • 02:11Making the Embryo Collection Cups
  • 02:31Adding GFP Flies to Embryo Collection Cups
  • 04:43Preparing a Mounting Chamber and Mounting Embryos
  • 07:15Imaging Embryos
  • 07:55Alternative Method: Mounting Embryos with Embryo -glue”
  • 09:30Results: Time-lapse Imaging of Cellularization
  • 10:34Conclusion

Summary

Automatic Translation

Automatic Translation

Early development of the fruit fly, Drosophila melanogaster, is characterized by a number of cell shape changes that are well suited for imaging approaches. This article will describe basic tools and methods required for live confocal imaging of Drosophila embryos, and will focus on a cell shape change called cellularization.

Tags

Imaging Cell Shape ChangeLiving Drosophila EmbryosConfocal ImagingGene ExpressionSignal TransductionCell PolarityCytoskeletal RemodelingMembrane TraffickingCellularizationPlasma Membrane GrowthSyncytial EmbryoCellular BlastodermEpithelial CellsMyosin-2 ContractilityExocytosisInternal Stores

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