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Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
This content is Free Access.
Chapters
- 00:05Title
- 01:10Introduction
- 03:52Cell Preparation and Seeding on Micropatterns
- 06:14Blebbistatin Treatment, Cell fixation and Immunofluorescent Staining
- 08:58Microscope Setup and Automated Image Acquisition
- 11:02Automated Image Processing and Analysis
- 13:40Representative Micropattern Imaging Results
- 14:47Conclusion
Adhesive micropatterns that normalize cellular architecture can be used to increase sensitivity in the detection of drug effects, improve reproducibility and simplify automated image acquisition and analysis. Such technology will benefit drug/siRNA screening assays, performed on conventional cell culture supports and consequently suffering from excessive cell-to-cell variability.
Tags
VisualizationQuantitative AnalysisDrug EffectsMicropatterned CellsHCA (High Content Analysis)Adherent Cell LinesHomogenous SubstrateCell ShapeMorphologyBehaviorCell-to-cell VarianceMicropatternsShape NormalizationInternal PolarityCYTOOMicropatterning TechnologyCell SeedingDrug TreatmentFixation And StainingAutomated AcquisitionAutomated Image ProcessingData AnalysisCell CytoskeletonActin Meshwork
