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Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)
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Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)

DOI:
10.3791/2518-v

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13:02 min

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March 18, 2011

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,
1Department of Biochemistry and Molecular Biology,Michigan State University

Chapters

  • 00:00Title
  • 00:50Introduction
  • 01:50Lipid Extraction
  • 02:49Thin Layer Chromatography (TLC)
  • 06:21Fatty Acyl Methylester (FAME) Reaction
  • 08:29Gas-Liquid Chromatography (GLC)
  • 10:21Representative Results for Polar Glycerolipid Profiling by TLC Coupled with GLC
  • 12:21Conclusion

Summary

Automatic Translation

Automatic Translation

Composition of polar lipid extracts and the fatty acid composition of individual glycerolipids are determined in a simple and robust lipid profiling experiment. For this purpose, glycerolipids are isolated by thin layer chromatography and subjected to transmethylation of their acyl groups. Fatty acyl methylesters are quantified by gas-liquid chromatography.

Tags

Arabidopsis ThalianaPolar Glycerolipid ProfilingThin Layer Chromatography (TLC)Gas-liquid Chromatography (GLC)Biological MembranesGlycerolipidsPhosphatidylcholinePhosphatidylethanolamineBilayer MembranesChemical ExchangeChloroplastGlycolipidsMonogalactosyldiacylglycerol (MGDG)Digalactosyldiacylglycerol (DGDG)Sulfoquinovosyldiacylglycerol (SQDG)PhotosynthesisCrystal StructuresPhotosystem I And IIPhosphate StarvationExtraplastidic MembranesPhospholipidsBiosynthesisGenetic StudiesBiochemical Studies

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