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Chapters
- 00:00Title
- 00:50Introduction
- 01:50Lipid Extraction
- 02:49Thin Layer Chromatography (TLC)
- 06:21Fatty Acyl Methylester (FAME) Reaction
- 08:29Gas-Liquid Chromatography (GLC)
- 10:21Representative Results for Polar Glycerolipid Profiling by TLC Coupled with GLC
- 12:21Conclusion
Composição de extratos de lipídios polares e da composição de ácidos graxos de glycerolipids individuais são determinados em um experimento simples e robusto perfil lipídico. Para este fim, glycerolipids são isolados por cromatografia em camada delgada e submetido a transmetilação de seus grupos acil. Graxos methylesters acila são quantificados por cromatografia gás-líquido.
Tags
Arabidopsis ThalianaPolar Glycerolipid ProfilingThin Layer Chromatography (TLC)Gas-liquid Chromatography (GLC)Biological MembranesGlycerolipidsPhosphatidylcholinePhosphatidylethanolamineBilayer MembranesChemical ExchangeChloroplastGlycolipidsMonogalactosyldiacylglycerol (MGDG)Digalactosyldiacylglycerol (DGDG)Sulfoquinovosyldiacylglycerol (SQDG)PhotosynthesisCrystal StructuresPhotosystem I And IIPhosphate StarvationExtraplastidic MembranesPhospholipidsBiosynthesisGenetic StudiesBiochemical Studies
