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JoVE Journal
Immunology and Infection

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Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
 

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Article DOI: 10.3791/2531
December 27th, 2010

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Summary December 27th, 2010

HIV tropism can be inferred from the V3 region of the viral envelope. V3 is PCR amplified in triplicate using nested RT-PCR, sequenced, and interpreted using bioinformatic software. Samples with with 1 or more sequence(s) with low g2P scores are classified as non-R5 virus.

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