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Passive Administration of Monoclonal Antibodies Against H. capsulatum and Others Fungal Pathogens
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Chapters
- 00:05Title
- 02:01Growth of H. capsulatum
- 03:50Hybridoma Growth and Purification of Monoclonal Antibodies
- 04:37Mouse Anesthesia and Intranasal Infections
- 06:22Survival Studies
- 07:17CFU Studies
- 08:27Monoclonal Antibody Protection Against H. capsulatum is Dependent on Antibody Specificity and Isotype
- 09:18Conclusion
C57BL/6 mice have been used to study Hc pathogenesis and provide the best model. We are exploring the potential benefits of humoral immunity against this fungus and generated several mAbs [to histone H2B and a heat shock protein 60kDa] that we tested for their protective efficacy after intraperitoneal administration.
Tags
Passive AdministrationMonoclonal AntibodiesH. CapsulatumFungal PathogensHistoplasmosisAntibody TherapyVirulence FactorsMAbs GenerationSolid Phase ELISA SystemSplenocytes FusionC57BL/6 MiceInfection AssessmentMortality As An EndpointColony Forming Units (CFU)Survival StudiesIntervention EfficacyVaccine EfficacyVirulence Testing
