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Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology
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Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology

Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology

DOI:
10.3791/2533-v

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12:50 min

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March 31, 2011

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, ,
1Department of Biology,Syracuse University, 2Department of Science Teaching,Syracuse University

Chapters

  • 00:05Title
  • 02:04Fixation of Embryos and Proteinase K Digestion
  • 05:34Hybridization of Riboprobes
  • 07:42Blocking and Anti-Digoxigenin (alpha-DIG) Antibody Incubation
  • 09:11Staining and Final Processing
  • 10:58Expression of aldh1a2, fgf8a, and pax2a in Zebrafish Embryos
  • 12:12Conclusion

Summary

Automatic Translation

Automatic Translation

Whole mount in situ hybridization (WISH) was used in an upper level undergraduate Comparative Vertebrate Biology course in addition to vertebrate dissections. This gave students the opportunity to study gene expression patterns as well as gross anatomy, linking the study of molecular and organismal biology within one course.

Tags

Whole Mount In Situ HybridizationGene ExpressionMRNA TranscriptsMolecular BiologyOrganismal BiologyComparative Vertebrate Biology LabEmbryologyGross AnatomyDissectionsModelsVertebrate DevelopmentZebrafish EmbryosFibroblast Growth Factor 8 A (fgf8a) Mutant LineRNA ProbesMidline ExpressionHeart DevelopmentSomitesTailbudMyotomeBrain DevelopmentStaining Reaction

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