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FISH for Pre-implantation Genetic Diagnosis
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FISH for Pre-implantation Genetic Diagnosis

Article DOI: 10.3791/2570 • 07:34 min • February 23rd, 2011
February 23rd, 2011 •
1, 1, 1
1Department of Cytogenetics, GSTS-Pathology, Guy’s & St Thomas’ NHS Foundation Trust, Guy’s & St Thomas’ Centre for Preimplantation Genetic Diagnosis

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This article describes the selection of suitable probes for single-cell FISH, spreading techniques for blastomere nuclei, and in situ hybridization and signal scoring, applied to pre-implantation genetic diagnosis (PGD) in a clinical setting.

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Pre-implantation Genetic Diagnosis PGD Assisted Reproduction Technology ART Monogenic Disease Cystic Fibrosis Chromosome Rearrangement Recurrent Miscarriages Infertility Ovarian Stimulation In Vitro Fertilization IVF Intracytoplasmic Sperm Injection ICSI Cleavage-stage Embryos Biopsy Fluorescence In Situ Hybridization FISH Chromosome Imbalance X-linked Disease Mutation-specific Test Chromosome Aneuploidy PGS
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