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A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures

A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures

DOI:
10.3791/2616-v

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05:02 min

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February 10, 2011

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, , , , , ,
1Department of Pathology and Laboratory Medicine,Cedars-Sinai Medical Cente, 2Pasadena, CA,Southern California Permanente Medical Group, 3Detroit,Detroit Medical Center, 4Woburn, MA,AdvanDx

Chapters

  • 00:05Title
  • 00:58Specimen Preparation
  • 01:20Gram Staining
  • 02:16PNA FISH Stain
  • 02:51Hybridization
  • 03:26Stringent Wash
  • 03:53Mounting
  • 04:10PNA FISH Based Identification of Enterococcus Species
  • 04:23Conclusion

Summary

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A rapid protocol for the direct identification of Enterococcus faecalis and other Enterococcus species from a positive blood culture using a Peptide Nucleic Acid fluorescent in situ hybridization assay (PNA FISH).

Tags

Enterococcus SpeciesBlood CulturesIdentification ProcedureRapid TestE. FaecalisE. FaeciumAmpicillin ResistanceVancomycin ResistanceSpecies DifferentiationTherapyResistance SurveillanceE. Faecalis OE PNA FISH AssayPeptide Nucleic Acid ProbesFluorescence In Situ HybridizationTime To ResultsSpecies-specific TreatmentMulticenter Studies1.5 Hour Procedure2.5 Hour Assay ProcedureStandard Bacteriology Methods

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