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胚肢の皮膚の血管系のためのホールマウント免疫組織化学的解析:胚における血管分岐形態形成を研究するモデルシステム
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JoVE Journal
Biology
Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
Please note that all translations are automatically generated.
Click here for the English version.
胚肢の皮膚の血管系のためのホールマウント免疫組織化学的解析:胚における血管分岐形態形成を研究するモデルシステム
DOI:
10.3791/2620-v
•
09:53 min
•
May 20, 2011
•
Wenling Li
,
Yoh-suke Mukouyama
1
Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center
,
National Heart, Lung, and Blood Institute, National Institutes of Health
Chapters
00:05
Title
01:42
Collecting Embryonic Limb Skins
03:53
Whole-mount Immunohistochemical Staining of Limb Skins
07:14
Mounting the Limb Skins on a Slide
08:24
Results of Whole-mount Confocal Immunofluorescence Microscopy
09:29
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
我々はマウス胚の四肢の皮膚に複雑な血管網の形成を分析するために複数のラベルで共焦点顕微鏡を走査型ホールマウントの免疫組織化学とレーザーをご紹介。
Tags
Whole-mount Immunohistochemical Analysis
Embryonic Limb Skin Vasculature
Vascular Branching Morphogenesis
Vascular Development
Limb Skin Vasculature Model
Whole-mount Confocal Microscopy
Fluorescent Markers
Genetic Studies
Molecular Mechanisms
Peripheral Nerves
Spatial Template
Differentiation And Patterning Of Arteries
Staining Intact Blood Vessels
Vascular Specific Antibodies
Fluorescent Secondary Antibodies
Vascularized Embryonic Organs
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