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JoVE Journal
Biology

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Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
 

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Article DOI: 10.3791/2627
May 29th, 2011

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Summary May 29th, 2011

We will describe a method which measures the kinetics of ion transport of membrane proteins alongside site-specific analysis of conformational changes using fluorescence on single cells. This technique is adaptable to ion channels, transporters and ion pumps and can be utilized to determine distance constraints between protein subunits.

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