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Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography
This content is Free Access.
Chapters
- 00:05Title
- 01:30Dual Hydrogel Constructs for Tissue Explant Cultures: DRG Explant Adhesion
- 02:49Dual Hydrogel Constructs for Tissue Explant Cultures: Dynamic Mask Photopolymerization
- 06:42Dual Hydrogel 3D Cell Encapsulation of Dissected Neurons
- 09:58Single Hydrogel 3D Cell Encapsulation of Dissociated Neurons
- 11:45Thin Film Hydrogel Polymerization
- 14:44Exampled of Undesirable Results
- 15:35Conclusion
Simple techniques are described for the rapid production of microfabricated neural culture systems using a digital micromirror device for dynamic mask projection lithography on regular cell culture substrates. These culture systems may be more representative of natural biological architecture, and the techniques described could be adapted for numerous applications.
Tags
Micropatterned HydrogelsNeural Culture SystemsDynamic Mask Projection PhotolithographyPatterned Cell Culture Environments3D EnvironmentsIn Vitro ExperimentsIn Vivo QualitiesCancer ResearchNeural EngineeringCardiac PhysiologyCell-matrix InteractionHydrogelsMolecular CompositionFunctionalizationElectrospinningElastomer StampsInkjet PrintingAdditive PhotopatterningStatic Photomask Projection-lithographyDynamic Mask MicrostereolithographyDigital Micromirror Device (DMD)Poly-(ethylene Glycol) (PEG) HydrogelsUV Initiated Free Radical Polymerization
