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Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
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Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

DOI:
10.3791/2662-v

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06:29 min

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March 01, 2011

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, , ,
1Translational Immunology Section, Office of Science and Technology,National Institute of Arthritis and Musculoskeletal and Skin Diseases, 2Translational Autoinflammatory Disease Section, Office of the Clinical Director,National Institute of Arthritis and Musculoskeletal and Skin Diseases

Chapters

  • 00:05Title
  • 01:29Collection of Whole Blood
  • 02:02Preparation of Whole Blood
  • 03:53Stimulation of the Whole Blood
  • 05:15Cytokine Assay
  • 05:34Over-Production of IL-1B in Whole Blood from Patients with Autoinflammatory Manifestations in Response to Stimulation with LPS in the Presence of ATP
  • 06:08Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a simple immunoassay to measure the production of pro-inflammatory cytokines, such as IL-1 beta production, in patients presenting with autoinflammatory phenotypes. By activating cells in whole blood cultures with pathogen-associated molecular patterns, specifically with lipopolysaccharide, cytokine secretion can be conveniently evaluated in whole blood supernatants.

Tags

Pro-inflammatory CytokineIL-1βWhole Blood Stimulation AssayInflammatory ProcessesImmune CellsCytokine HomeostasisInnate Immune SystemPathogensDanger StimuliInterleukin-1 (IL-1)SecretionAutoinflammatory SyndromesLPSCellular Immune ResponsesPAMPsGram-negative Bacterial Endotoxin

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