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Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)

Characterizing Bacterial Volatiles using Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)

DOI:
10.3791/2664-v

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08:54 min

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June 08, 2011

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, ,
1School of Engineering,University of Vermont

Chapters

  • 00:05Title
  • 02:18Biological Experiment: Set-up and Safety Considerations
  • 03:25Turning on and Tuning the SESI-MS for Analysis
  • 06:12Obtaining a Volatile Fingerprint of Your Bacterial Culture
  • 07:35Bacterial VOC Fingerprints
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

Secondary electrospray ionization mass spectrometry (SESI-MS) enables the detection of volatile organic compounds (VOCs) without the need for any sample pretreatment. This protocol provides instructions for the rapid (within minutes) characterization of bacterial VOCs using SESI-MS.

Tags

Secondary Electrospray Ionization Mass Spectrometry (SESI-MS)Volatile CompoundsRapid DetectionSample PretreatmentIllicit DrugsExplosivesSkin VolatilesBreath AnalysisProton Transfer ReactionsHetero-organic MoleculesOrganic VolatilesAerosolsBacterial VolatilesBacterial IdentificationBacterial CharacterizationVolatile FingerprintingStatistical Analysis MethodBacterial GeneraBacterial SpeciesMixed CulturesGrowth Media

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