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Chapters
- 00:05Title
- 01:46Preparation
- 03:56Generating HUVEC Monolayer
- 05:52Addition of Invading Cells and Data Normalization
- 07:27Representative Invasion Assay Results
- 08:18Conclusion
Questo articolo descrive una<em> In vitro</em> Tecnica per le cellule tumorali che invadono monitoraggio mediante un monostrato di cellule endoteliali. I dati vengono acquisiti in tempo reale in funzione delle variazioni dell'impedenza sulla superficie degli elettrodi sul fondo del pozzo.
Tags
Real-timeElectrical ImpedanceTechniqueMeasureInvasionEndothelial Cell MonolayerCancer CellsMetastatic DisseminationBasement Membrane DegradationTumor Cell AttachmentVascular EndotheliumEndothelial Junction RetractionInvasion And MigrationCirculatory SystemAdherence To Capillary WallsMetastatic Tumors FormationTumor Cell-endothelial Cell InteractionIn Vitro ReplicationMonolayer Of Human Umbilical Vein Endothelial Cells (HUVEC)Cancer Cell ChallengeElectron MicroscopyPhase-contrast MicroscopyIn Vivo Metastatic Process RepresentationElectrical Impedance Fluctuations MeasurementXCELLigence InstrumentRoche Manufacturing
