Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

Steven Robbins; Jisha Jacob; Xinxin Lu; Mary Ann Moran; Xiaozhen Mou

doi:10.3791/2855

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Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

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Biology

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Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

20,958 Views

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12:46 min

•

September 10, 2011

DOI:

10.3791/2855-v

DOI:

10.3791/2855-v

•

12:46 min

September 10, 2011

•

2 Views

Steven Robbins*¹, Jisha Jacob*¹, Xinxin Lu¹, Mary Ann Moran², Xiaozhen Mou¹

¹Biological Sciences,Kent State University, ²Marine Sciences,University of Georgia (UGA)

Summary

Environmental bacterioplankton are incubated with a model dissolved organic carbon (DOC) compound and a DNA labeling reagent, bromodeoxyuridine (BrdU). Afterward, DOC-degrading cells are separated from the bulk community based on their elevated BrdU incorporation using fluorescence activated cell sorting (FACS). These cells are then identified by subsequent molecular analyses.