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Biology

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ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells
 

ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells

Article DOI: 10.3791/2857 12:20 min
August 31st, 2011

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Summary August 31st, 2011

The biarsenical dyes FlAsH and ReAsH bind specifically to tetracysteine motifs in proteins and can selectively label proteins in live cells. Recently this labeling strategy has been used to develop sensors for different protein conformations or oligomeric states. We describe the labeling approach and methods to quantitatively analyze binding.

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