A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 00:18Introduction
- 01:52The SU-8 Master
- 02:51PDMS Replica Molding
- 07:05Preparing the Thin PDMS Valve Layer
- 09:11Plasma Bonding Control and Valve Layers
- 10:37Aligning Control and Fluidic Layers
- 12:02Computer-controlled Opening and Closing of PDMS Microvalves by Vacu
- 15:09An Integrated Microfluidic System for Computer Controlled Perfusion
- 17:32Conclusion
We demonstrate protocols for manufacturing and automating elastomeric polydimethylsiloxane (PDMS)-based microvalve arrays that need no extra energy to close and feature photolithographically defined precise volumes. A parallel subnanoliter-volume mixer and an integrated microfluidic perfusion system are presented.
Tags
Microfluidic ChipsElastomeric Microvalve ArraysPoint-of-care DiagnosticsHigh-throughput Biomedical AssaysFlow ControlFluidic VolumesPolydimethylsiloxane (PDMS)Portable DevicesDeep ChannelsVertical SidewallsPrecise FeaturesThree-layered DevicesFluidic LayerControl LayerThin PDMS MembraneReplica MoldingSU-8 Photoresist MastersOxygen ActivationSolenoid ValvesComputer Software
