Name: BioMEMS and Cellular Biology: Perspectives and Applications (Video) | JoVE Journal
Uploaded: 2007-10-01T00:00:00.000+00:00
Duration: 16 min 30 s
Description: Explore microfluidic techniques for neuroscience research using BioMEMS technology. This method enables precise control of cellular environments to study neuron behavior and axon guidance in vitro.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

playerTrialDesktop

biomems and cellular biology: perspectives and applications

BioMEMS and Cellular Biology: Perspectives and Applications

The ability to culture cells has revolutionized hypothesis testing in basic cell and molecular biology research. It has become a standard methodology in drug screening, toxicology, and clinical assays, and is increasingly used in regenerative medicine. However, the traditional cell culture methodology   essentially consisting of the immersion of a large population of cells in a homogeneous fluid medium and on a homogeneous flat substrate   has become increasingly limiting both from a fundamental and practical perspective. Microfabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, and the medium composition, as well as the neighboring cell type in the surrounding cellular microenvironment. Additionally, microtechnology is conceptually well-suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. In this interview, Albert Folch explains these limitations, how they can be overcome with soft lithography and microfluidics, and describes some relevant examples of research in his lab and future directions.

The ability to culture cells has revolutionized hypothesis testing in basic cell and molecular biology research. It has become a standard methodology in ...

I'm Albert Falk. I'm a professor at the University of Washington in Seattle, and I work on the broad area of biomes, which stands for biomedical micro electromechanical systems. And we work on microfluidics and we focus on applications in cell biology, primarily in in neuroscience.One of the focuses of the lab is to try to overcome the limitations of traditional cell culture technology. If you look at the, the, the way cell biologists study cells in, in vitro in a, in a Petri dish, you will notice immediately that the cells are bathed in a homogeneous cell culture medium, and they're also sitting on a, on a surface that is homogeneous. However, in the body cells are not on a homogeneous surface.They're not bathed in, in a homogeneous medium. They're actually exposed to a variety of, of signals that change in space and time, often the form of gradients, and they are surrounded by a, by a gel type of matrix. There are also some practical limitations when you study cells in a Petri dish, you need an incubator, which is a, a large and expensive piece of equipment.Typically, biologists want to study a bunch of different conditions. They want to study cells in a, let's say in a cell cult

biomems-and-cellular-biology-perspectives-and-applications

Research

JoVE Journal

Biology

Using Micro-Electro-Mechanical Systems (MEMS) to Develop Diagnostic Tools

Micro-scale Engineering for Cell Biology

This video describes the fabrication and use of a microfluidic device to culture central nervous system (CNS) neurons.  This device is compatible with ...

BioMEMS: Forging New Collaborations Between Biologists and Engineers

Here we present a protocol to micropattern cells at single-cell resolution using DNA-programmed adhesion. This protocol uses a benchtop photolithography platform to create patterns of DNA oligonucleotides on a glass slide and then labels cell membranes with commercially available complementary oligonucleotides. Hybridization of the oligos results in programmed cell adhesion.

Simple, Affordable, and Modular Patterning of Cells using DNA

Well-characterized genetic parts are necessary for the design of novel genetic circuits. Here we describe a cost-effective, high-throughput method for rapidly characterizing genetic parts. Our method reduces cost and time by combining cell-free lysates, linear DNA to avoid cloning, and acoustic liquid handling to increase throughput and reduce reaction volumes.

Rapid Characterization of Genetic Parts with Cell-Free Systems

The present protocol describes the design, fabrication, and characterization of a microfluidic system capable of aligning, immobilizing, and precisely compressing hundreds of Drosophila melanogaster embryos with minimal user intervention. This system enables high-resolution imaging and recovery of samples for post-stimulation analysis and can be scaled to accommodate other multicellular biological systems.

Mechanostimulation of Multicellular Organisms Through a High-Throughput Microfluidic Compression System

Membrane lipid diversity in structure and composition is an important contributor to cellular processes and can be a marker of disease. Molecular dynamics simulations allow us to study membranes and their interactions with biomolecules at atomistic resolution. Here, we provide a protocol to build, run, and analyze complex membrane systems.

Realistic Membrane Modeling Using Complex Lipid Mixtures in Simulation Studies

The success of single-cell/single-nuclei transcriptomics and multi-omics largely depends on the quality of cells/nuclei. Therefore, isolating cells/nuclei from tissue and their purification must be highly standardized. This protocol describes the preparation of nuclei from the brain and bone marrow for downstream single-nuclei multiome assay.

High-Quality Brain and Bone Marrow Nuclei Preparation for Single Nuclei Multiome Assays

Biology of Microbial Communities  - Interview

Perspectives on Neuroscience

Explore microfluidic techniques for neuroscience research using BioMEMS technology. This method enables precise control of cellular environments to study neuron behavior and axon guidance in vitro.

BioMEMS and Cellular Biology: Perspectives and Applications

Explore More Videos