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Journal / Biology / Isolering og karakterisering af RNA-Varer Exosomes
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Isolering og karakterisering af RNA-Varer Exosomes
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Isolering og karakterisering af RNA-Varer Exosomes

Article DOI: 10.3791/3037-v • 09:44 min • January 9th, 2012
January 9th, 2012 •
*1, *1, 1
1Krefting Research Centre, Department of Internal Medicine, Sahlgrenska Academy, University of Gothenburg
* These authors contributed equally

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Dette papir demonstrerer metoder til isolering, oprensning og detektion af exosomes, samt teknikker til analyse af deres molekylære indhold. Disse metoder er tilpasses til exosome isolation fra både cellekulturmedier og biologiske væsker, og kan udover analyse af molekylære indhold også være nyttige i funktionelle studier.

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Molekylær Biologi Exosomes microvesicles mRNA miRNA RNA isolation flowcytometri elektronmikroskopi Western blot Bioanalyzer
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