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Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

DOI:
10.3791/3041-v

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12:38 min

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August 09, 2011

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, ,
1Department of Structural Biology,University of Pittsburgh School of Medicine

Chapters

  • 00:05Title
  • 02:04Frozen-hydrated Electron Microscopy (EM) Specimen Preparation
  • 03:22Cryo-EM of HIV-1 Capsid Protein Tubular Assemblies
  • 05:26Helical Indexing
  • 07:13Three-dimensional Reconstruction
  • 10:42Representative Results: HIV-1 Capsid Assemblies Structure Determination
  • 12:16Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a method to obtain a three-dimensional (3D) structure of helically assembled molecules using cryo-electron microscopy. In this protocol, we use HIV-1 capsid assemblies to illustrate the detailed 3D reconstruction procedure for achieving a density map by the iterative helical real-space reconstruction method.

Tags

HIV-1Capsid AssembliesCryo-electron MicroscopyIterative Helical Real-space ReconstructionMacromolecular Protein ComplexesImage ProcessingSingle Particle Electron MicroscopyTwo-dimensional Electron CrystallographyHelical ObjectsIHRSR MethodFilamentous FormsActin FilamentsMicrotubulesAmyloid FibersTobacco Mosaic VirusesBacteria Flagella3D Density MapFlexible And Disordered Helical AssembliesVideo ArticleProtocols

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