Tumor Implantation and Injection of a Tumor Vaccine in a Mouse Model

Take an anesthetized and shaved mouse. Inject mouse skin cancer cells.

Due to the same genetic background, the host immune system does not reject the cancer cells, allowing them to grow into a tumor.

Measure the tumor dimensions periodically.

Take a tumor vaccine containing non-dividing skin cancer cells modified to secrete a cytokine that activates immune cells against the tumor.

Once the tumor grows to the desired size, inject the vaccine near the tumor.

The secreted cytokine binds to specific immune cells in the tumor region, triggering an immune signaling cascade.

This process recruits more immune cells to the tumor site.

Together, they target and destroy the cancer cells, suppressing the tumor growth.

Intradermally implant 500,000 cells B16-F10 tumor cells in 50 microliters of cold PBS, and the left flank using a 30-gauge needle. The dose of implanted B16-F10 tumor cells may need to be adjusted in a range of 50,000 to 500,000 cells for successful tumor development.

After implementation, measure the tumor length and width three times a week using an electronic digital caliper. Calculate the tumor volume, and treat the mice with tumor vaccine when tumors have reached a size of 2