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Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

Article DOI: 10.3791/3129-v • 08:20 min • October 17th, 2011
October 17th, 2011 •
1, 1, 1,2, 1,3
1Department of Biomedical Engineering, Washington University, 2Institute for Information Transmission Problems, Russian Academy of Sciences, 3Department of Mechanical Engineering and Materials Science, Washington University

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This article describes surface labeling and ex ovo tissue culture in the early chick embryo. Techniques amenable to time-lapse bright field, fluorescence, and optical coherence tomography imaging are presented. Tracking surface labels with high spatiotemporal resolution enables kinematic quantities such as morphogenetic strains (deformations) to be calculated in both two and three dimensions.

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Morphogenetic Tissue Deformations Early Chick Embryo Embryonic Epithelia Bending Twisting Folding Stretching Primitive Organs Tracking Fiducial Markers Morphogenetic Quantities Growth Contraction Shear Surface Labeling Techniques Imaging Modalities Fluorescent Labels Magnetic Iron Particles 2-D Tissue Deformations Polystyrene Microspheres Contrast Agents Non-invasive Optical Coherence Tomography (OCT) Imaging 3-D Tissue Deformations Physical Mechanisms Head Fold Development Heart Development Brain Development
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