Simultaneous Plethysmography and Electromyography Recording in an ALS Mouse Model

Begin with a mouse with amyotrophic lateral sclerosis or ALS, a motor neuron degenerative disease.

The mouse carries an implanted transmitter with electrode connections inserted into the scalene and trapezius muscles, which are involved in breathing.

Place this mouse in a plethysmography or a respiratory chamber positioned over a receiver and allow it to move freely.

In the chamber, the airflow is continuously monitored during the mouse's breathing while resting or moving and recorded as plethysmography signals.

Bring a magnet near the mouse to activate the transmitter, turning the receiver light on and confirming electrode activation.

These electrodes detect the electrical signals from the muscles and transmit these electromyography or EMG signals wirelessly to the recording device.

Compare the plethysmography and EMG signals from both muscles to analyze the muscle activity during breathing in resting and moving states in the ALS mouse.

Place the mouse in the plethysmography chamber for at least one hour to acclimate it prior to recording EMG and plethysmography. Prior to recording, but after calibration, turn on the transmitter by placing a strong magnet within one inch of the impla