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JoVE Journal
Biology

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A Quantitative Assay for Insulin-expressing Colony-forming Progenitors
 

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

Article doi: 10.3791/3148
November 28th, 2011

Summary November 28th, 2011

A three-dimensional clonogenic assay that allows pancreatic-like progenitors to differentiate into insulin-expressing colonies is described. This method takes advantage of semi-solid media containing methylcellulose, Matrigel and growth factors, in which single progenitors proliferate and differentiate in vitro, permitting quantification of the number of functional progenitors in a population.

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