JoVE Encyclopedia of Experiments
Neuroscience
0 views • 5:12 min • April 28th, 2025
Begin with a secured anesthetized rat pup on a heating pad.
Sterilize the cervical area alternately with ethanol and povidone-iodine. Administer local anesthesia at the incision site.
Make a cervical incision, exposing the common carotid artery.
Place retractors to ensure clear access.
Identify the internal carotid artery or ICA and remove surrounding tissue.
Tie a ligature around the base of the ICA and retract it to temporarily halt blood flow.
Place a loose second ligature on the ICA.
Make an incision between the ligatures.
Take a silicone-coated nylon occlusion suture and insert it through the incision until it reaches the middle cerebral artery or MCA.
This blocks the blood flow to the MCA, inducing local ischemia in the brain.
Tie the ligature around the occlusion suture to secure it.
Close the incision site. Allow the pup to recover before further studies.
After anesthetizing the pup using inhaled isoflurane according to approved protocols, ensure that there is no response to a foot pinch. Maintain body surface temperature with the use of a heating pad under the surgical stage. Secure the animal in a supine position with tape across the shoulder regions.
Use sterile cotton swabs to sw
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