In this protocol, we describe the dissection of placentae from the mouse on pregnancy d10.5, followed by isolation of trophoblast cells using a Percoll gradient. We then demonstrate use of the isolated cells in a matrigel invasion assay.
Pennington, K. A., Schlitt, J. M., Schulz, L. C. Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay. J. Vis. Exp. (59), e3202, doi:10.3791/3202 (2012).