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Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting
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JoVE Journal Biology
Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting

Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting

DOI:
10.3791/3244-v

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13:41 min

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October 17, 2011

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, , , ,
1Department of Chemistry,Hunter College , 2Department of Biochemistry,Albert Einstein College of Medicine

Chapters

  • 00:05Title
  • 02:24RNA Preparation for Footprinting Experiment
  • 04:24The Footprinting Experiment
  • 08:09Data Analysis
  • 10:38Analysis of RNA Fragments after Peroxidative Fenton Footprinting Reaction
  • 12:23Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to quantify the Mg(II)-dependent formation of RNA tertiary structure by two methods of hydroxyl radical footprinting.

Tags

MonitoringEquilibrium ChangesRNA StructurePeroxidativeOxidativeHydroxyl Radical FootprintingRNA MoleculesGenetic InformationTertiary StructuresBiologic RoleRegulatorBinderCatalystNucleic AcidsPhosphodiester BackboneSingle Nucleotide ResolutionIntermolecular Contact SurfaceEquilibrium TransitionsKinetic TransitionsSolution VariableTimeProbe ExposurePolymer SamplingVideo ArticleSample PreparationMg(II)-mediated Folding Isotherms

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