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Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies
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Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

DOI:
10.3791/326-v

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11:52 min

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October 01, 2007

•

,
1Department of Physiology and Biophysics,University of California, Irvine (UCI)

Chapters

  • 00:01Title
  • 00:09Introduction
  • 02:08Preparing Reagents
  • 03:00Incubating Human Blood with RosetteSep NK Enrichment Cocktail
  • 05:15Isolating NK Cells after Histopaque Density Gradient Centrifugation
  • 11:07Conclusion

Summary

Automatic Translation

Automatic Translation

Natural killer cells are a small population of lymphocytes. Here we show how to isolate these cells from human blood by negative selection, using a kit from StemCell Technologies. The cells obtained are viable and untouched by antibodies, and therefore ready to be used for a number of procedures.

Tags

NK CellsRosetteSep KitStemCell TechnologiesPositive SelectionNegative SelectionSurface MarkersPeripheral BloodCytotoxic LymphocytesInnate Immune SystemCancerInfectionsPregnancyTransplant RejectionAntibody CocktailUnwanted Cell ActivationDifferentiationVideo-protocol

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