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Immunology and Infection
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Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

DOI:
10.3791/3263-v

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10:22 min

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September 16, 2011

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, , , , , ,
1Department of Molecular Biology,University of Salzburg, 2Department of Neurology,Paracelsus Medical University, 3Department of Dermatology,Paracelsus Medical University, 4Bühlmann Laboratories, 5Christian Doppler Laboratory for Allergy Diagnosis and Therapy,University of Salzburg

Chapters

  • 00:05Title
  • 01:46Preparation of Drug Conjugates
  • 03:11Determining the Coupling Rate of Drug Conjugates
  • 04:40Basophil Activation Test (BAT) Using Drug Conjugates
  • 07:32BAT is an Effective Tool for Determining IgE-dependent Drug Hypersensitivity
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described.

Tags

Basophil Activation TestInvestigationIgE-Mediated MechanismsDrug HypersensitivityNon-steroidal Anti-inflammatory DrugsPropyphenazoneDiclofenacType I-like Allergic ReactionsClinical PracticePatient HistorySkin TestingOral Provocation TestingIgE-mediated PathomechanismIn Vitro MethodHuman BasophilsDrug-allergic PatientsIgE Receptor CrosslinkingAllergic Effector MoleculesCD63 Surface ExpressionFlow CytometryLow Molecular Weight DrugsConjugatesHuman Serum Albumin (HSA)Carboxyl Group

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