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Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
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JoVE Journal Biology
Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)

DOI:
10.3791/3304-v

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09:11 min

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June 14, 2012

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, , , , ,
1Emerging Technologies,Sigma Life Science

Chapters

  • 00:05Title
  • 01:36Initiating the ZFN Design Process
  • 03:23Custom ZFN Production and Validation
  • 04:16Delivery of Validated ZFNs by Nucleofection
  • 06:25Cel-1 Assay
  • 07:09Clonal Isolation and Characterization
  • 07:46Estimated ZFN Activity from the Cel-1 Assay
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

The CompoZr Custom Zinc-Finger Nuclease (ZFN) Service enables precise genome editing in any organism or cell line at any locus defined by the user. This article describes the process for the design, manufacture, validation and implementation of the CompoZr Custom ZFN Service.

Tags

Genome EditingCompoZr Custom Zinc Finger NucleasesGene FunctionGenetic Basis Of DiseaseTraditional Gene Editing MethodsChemical-based MutagenesisRandom Integration Of DNA SequencesSynthetic SequencesAberrant Culture ConditionsTransient ZFN ExpressionPrecise Gene EditingHighly Efficient MannerZinc Finger NucleasesDouble-stranded DNA (dsDNA)CompoZr ZFN UnitDNA-cleavage DomainsDouble-strand Break (DSB)Non-homologous End-joining (NHEJ) DNA Repair PathwaySmall Insertions And Deletions (indels)FrameshiftFunctional Knockout

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