Here we use a human LentiPlex pooled library and traditional sequencing methods to identify gene targets promoting cell survival. We demonstrate how to set up and deconvolute a LentiPlex screen and validate the results.
Coussens, M. J., Corman, C., Fischer, A. L., Sago, J., Swarthout, J. MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells. J. Vis. Exp. (58), e3305, doi:10.3791/3305 (2011).