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09:12 min
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December 21, 2011
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Summary
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Here we use a human LentiPlex pooled library and traditional sequencing methods to identify gene targets promoting cell survival. We demonstrate how to set up and deconvolute a LentiPlex screen and validate the results.
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Cite this Article
Coussens, M. J., Corman, C., Fischer, A. L., Sago, J., Swarthout, J. MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells. J. Vis. Exp. (58), e3305, doi:10.3791/3305 (2011).
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