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JoVE Journal
Biology

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MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells
 

MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

Article DOI: 10.3791/3305
December 21st, 2011

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Summary December 21st, 2011

Here we use a human LentiPlex pooled library and traditional sequencing methods to identify gene targets promoting cell survival. We demonstrate how to set up and deconvolute a LentiPlex screen and validate the results.

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