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Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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Immunology and Infection
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JoVE Journal Immunology and Infection
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

DOI:
10.3791/3322-v

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15:17 min

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September 25, 2011

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, , , , ,
1School of Molecular Bioscience,University of Sydney, 2Department of Surgery,Royal Prince Alfred Hospital, 3Department of Anatomical Pathology,Department of Anatomical Pathology, 4Department of Medicine,Concord Repatriation General Hospital

Chapters

  • 00:05Title
  • 02:00Introduction
  • 03:38Sample Digestion
  • 04:39Sample Filtration
  • 06:32Sample Preparation for Cell Capture
  • 08:18Antibody Microarray Cell Capture
  • 10:41Fluorescence Multiplexing with Conjugated Antibodies
  • 12:38Results
  • 14:37Conclusion

Summary

Automatic Translation

Automatic Translation

We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.

Tags

Colorectal CancerCell Surface Protein ProfilingAntibody MicroarrayFluorescence MultiplexingCRC PrognosisProtein ExpressionPost-translational ModificationCluster Of Differentiation AntigensBiomarkersImmunohistochemistry (IHC)Cancer Sub-classificationPrognosticationMarkerClinico-pathological StagingProteomic TechniquesITRAQDiagnostic Laboratories

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