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In Situ Hybridization for the Precise Localization of Transcripts in Plants
JoVE Journal
Biology
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JoVE Journal Biology
In Situ Hybridization for the Precise Localization of Transcripts in Plants

In Situ Hybridization for the Precise Localization of Transcripts in Plants

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12:15 min

November 23, 2011

DOI:

12:15 min
November 23, 2011

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Summary

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The in situ hybridization protocol described here allows a direct localization of mRNA and small RNA expression at the cellular level with high sensitivity and specificity. The procedure is optimized for paraffin-embedded plant tissue sections, is applicable to a wide range of plants and tissues, and can be completed within ten days.

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