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JoVE Journal
Biology

This content is Open Access.

In Situ Hybridization for the Precise Localization of Transcripts in Plants
 

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Article DOI: 10.3791/3328
November 23rd, 2011

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Summary November 23rd, 2011

The in situ hybridization protocol described here allows a direct localization of mRNA and small RNA expression at the cellular level with high sensitivity and specificity. The procedure is optimized for paraffin-embedded plant tissue sections, is applicable to a wide range of plants and tissues, and can be completed within ten days.

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