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Chapters
- 00:05Title
- 01:42Tissue Embedding
- 02:36Sectioning of Tissue Blocks
- 04:14In Situ Hybridization
- 06:30Post-hybridization Treatment
- 08:27Representative Results of In Situ Hybridization
- 11:47Conclusion
The in situ hybridization protocol described here allows a direct localization of mRNA and small RNA expression at the cellular level with high sensitivity and specificity. The procedure is optimized for paraffin-embedded plant tissue sections, is applicable to a wide range of plants and tissues, and can be completed within ten days.
Tags
In Situ HybridizationPrecise LocalizationTranscriptsPlantsGenomics ResearchPlant BiologyGene ExpressionMicroarrayNext Generation SequencingDevelopmental ProcessesPhysiological ProcessesStress Response ProcessesEpigenetic PathwaysSmall RNA PathwaysGene Regulatory NetworksSpatiotemporal ResolutionProfiling MethodLaser MicrodissectionFluorescence-activated Cell SortingBiological Role Of A GeneSpatiotemporal Pattern Of ExpressionCellular ResolutionDevelopmentEnvironmental StimuliMutants AnalysisTransgenic Reporter Lines
