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Chapters
- 00:05Title
- 02:05Cloning of the Target Gene/ABDz1-tagged Fusion Construct
- 04:02Protein Expression
- 04:49Orthogonal Affinity Purification
- 08:04Results: SDS-PAGE Analysis of Purified Protein Products
- 09:45Conclusion
A novel and highly efficient two-step affinity chromatography protocol has been developed and is described in detail. The method is based on a small purification tag with two inherent affinities and is applicable to a wide range of target proteins with different properties.
Tags
Orthogonal Protein PurificationSmall Bispecific Affinity TagRecombinant ProteinsPurification ProtocolsHigh-throughput MethodsPurification TagsHexa-histidine TagAffinity ChromatographyBispecific Purification TagAlbumin-binding DomainStreptococcal Protein GHuman Serum Albumin (HSA)Phage Display TechnologyDimeric Z-domainStaphylococcal Protein A
