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JoVE Journal
Biology

This content is Open Access.

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
 

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Article DOI: 10.3791/3370
January 16th, 2012

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Summary January 16th, 2012

A novel and highly efficient two-step affinity chromatography protocol has been developed and is described in detail. The method is based on a small purification tag with two inherent affinities and is applicable to a wide range of target proteins with different properties.

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