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Chapters
- 00:05Title
- 02:42A Two-Chamber Assay for Frog Sperm Chemotaxis
- 05:46Representative Evaluation of the Chemoattractiveness of Frog Egg Water Proteins
- 08:22Results: Evaluation of Sperm Trafficking Patterns and Behaviors in Response to a Chemotactic Stimulus
- 09:40Conclusion
Æg og det ekstracellulære belægninger omkring æg ofte release peptider, proteiner og små molekyler, der kommunikerer med sædceller til at guide dem til ægget derved at fremme befrugtningen. Brug af frøen sædceller vi beskrive og sammenligne to klasser af assays anvendes til at påvise sædceller chemoattraction - sperm ophobning analyser og sperm sporing assays.
Tags
AssaysDetectingFrog SpermChemoattractionInvertebratesVertebratesQuantitative AssaysSperm MovementSperm Accumulation AssaysSwimming TrajectoriesDose Response CurvesTime CoursesNeutrophil ChemotaxisBacterial PeptidesFollicular FluidSperm Tracking AssaysLabor IntensiveSwimming PathsChemoattractant Gradient AxisCharacteristic TurnsChanges In Orientation
