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JoVE Journal
Biology

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Two Types of Assays for Detecting Frog Sperm Chemoattraction
 

Two Types of Assays for Detecting Frog Sperm Chemoattraction

Article DOI: 10.3791/3407
December 27th, 2011

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Summary December 27th, 2011

Eggs and the extracellular coatings around eggs frequently release peptides, proteins and small molecules that communicate with sperm to guide them to the egg thereby promoting fertilization. Using frog sperm we describe and compare two classes of assays used to detect sperm chemoattraction – sperm accumulation assays and sperm tracking assays.

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