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1Department of Physiology and Biophysics, Confocal Microscopy and Cell Imaging Core,Robert Wood Johnson Medical School , 2Department of Physiology and Biophysics,Robert Wood Johnson Medical School , 3Muscle Biology Research Group-MUBIG Schools of Nursing & Medicine,University of Missouri-Kansas City
Chapters
- 00:05Title
- 02:11Intracellular Ca2+ Measurement for Individual Cells
- 04:45Mn2+ Quenching Assay in Cultured Cells
- 05:20Mn2+ Quenching Assay in Muscle Cells
- 06:40Spatially and Temporally Resolved SOCE in Skinned Muscle Fibers
- 10:20SOCE Activity in KYSE-150 and Muscle Cells
De omvang van de winkel bediende Ca<sup> 2 +</sup> Entry (SOCE) kan worden gecontroleerd met behulp van fluorescerende Ca<sup> 2 +</sup> Indicatoren. Mn<sup> 2 +</sup> Quenching van dergelijke indicatoren testen SOCE in gekweekte cellen en skeletspieren vezels. Een techniek waarmee ruimtelijke en temporele resolutie van SOCE door confocale beelden van mechanisch gevild spiervezels wordt ook beschreven.
Tags
Fluorescence-based MeasurementStore-operated Calcium EntryLive CellsCultured Cancer CellSkeletal Muscle FiberSOCECapacitative Ca2+ EntryEndoplasmic ReticulumSarcoplasmic ReticulumSecond MessengerCellular ProcessesProliferationApoptosisGene TranscriptionMotilityEpithelial CellsPatch-clamp StudiesIntracellular Ca2+ MeasurementsHigh-throughput ScreeningFluorescence Methods
