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Chapters
- 00:05Title
- 01:01Fission Yeast Culture and Sample Preparation
- 03:28Microscopy and Quantitative Measurements
- 05:03Representative Measurements in Yeast
- 06:27Conclusion
The fission yeast, Schizosaccharomyces pombe, is a good model system to study basic cellular processes. Here we describe a method to perform quantitative live cell analysis of fission yeast. In this particular experiment we focus on organisation of the genome within the cell nucleus, but the method can also be used to study cytosolic factors.
Tags
Quantitative Live Cell Fluorescence-microscopy AnalysisFission YeastMicroscopy TechniquesSpecific Protein DetectionLive Cell ImagingFluorochromesGreen Fluorescent Protein (GFP)Subcellular LocalizationsProtein MovementsFluorescent MicroscopeSubnuclear LocalizationChromosome StudyLac Repressor Protein (LacR)LacO SequenceGenome VisualizationHomologous RecombinationTargeted IntegrationEngineered Fusion Proteins
